neuVitro
Professional
collagen coating protocol
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Professional
collagen coating procedure (both sides are equally coated):
Wash coverslips
2 hours.
Rinse
coverslips 6 times x 30 minutes each.
Treat
coverslips in 100% Ethanol 2 hour.
Treat
coverslips in 70% concentrated Nitric Acid 24 hours.
Rinse
coverslips in dd-water 6 times x 1 hour each.
Coat
coverslips 1mg/ml (primary coating) 24 hours at 4°C.
Rinse
coverslips in dd water 2 times x 10 minutes.
Dry
coverslips in hood for 12 hours.
Coat
coverslips 1mg/ml (secondary coating) 24 hours at 4°C.
Rinse
coverslips in buffer 2 times x 10 minutes. Dry
coverslips in hood for 12 hours.
Seal
coated coverslips into bags, 15
units/bag.
Sterilize
coverslips by
radiation.
Test
samples of coated coverslips in cell culture.
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Neuvitro
Corporation
The
collagen coating expert
with
G3 Digital Collagen Coating System
Ready
to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell
culture, starting $55/box
Ready
to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell
culture, starting $65/box
Ready
to use German coverslip with fibronectin coating on both sides for cell
culture, starting $89/box
Ready
to use German coverslip with human Laminin coating on both sides for cell
culture and neuron culture, starting $94/box
Ready
to use German coverslip with porcine gelatin coating on both sides for cell
culture, starting $89/box
CE
certified, tested for primary cell and primary neuron cultures, starting
$12/box
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