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collagen-coating-protocol

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 Professional collagen coating protocol

  

 

How to know if my coating protocol is good or not?

Collection of collagen coating protocols

View and compare multiple collagen coating protocols in one table

 

How to sterilize collagen?

 

Professional collagen coating procedure (both sides are equally coated):

 

Wash coverslips 2 hours.

Rinse coverslips 6 times x 30 minutes each.

Treat coverslips in 100% Ethanol 2 hour.

Treat coverslips in 70% concentrated Nitric Acid 24 hours.

Rinse coverslips in dd-water 6 times x 1 hour each.

Coat coverslips 1mg/ml (primary coating) 24 hours at 4C.

Rinse coverslips in dd water 2 times x 10 minutes.

Dry coverslips in hood for 12 hours.

Coat coverslips 1mg/ml (secondary coating) 24 hours at 4C.

Rinse coverslips in buffer 2 times x 10 minutes.

Dry coverslips in hood for 12 hours.

Seal coated coverslips into bags, 15 units/bag.

Sterilize coverslips by radiation.

Test samples of coated coverslips in cell culture.

Note:

This protocol is for automated coating machine only. Not suitable for lab.

 

 

 

Neuvitro Corporation

The collagen coating expert  

 

with G3 Digital Collagen Coating System

 

 

Poly-D-lysine coating

Pre-coated coverslips

Ready to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell culture, starting $55/box

 

Rat tail Collagen

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Ready to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell culture, starting $65/box

 

Fibronectin coating

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Ready to use German coverslip with fibronectin coating on both sides for cell culture, starting $89/box

 

Laminin coating

Pre-coated coverslips

Ready to use German coverslip with human Laminin coating on both sides for cell culture and neuron culture, starting $94/box

 

Gelatin coating

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Ready to use German coverslip with porcine gelatin coating on both sides for cell culture, starting $89/box

 

German Coverslips 

Cell culture tested

CE certified, tested for primary cell and primary neuron cultures, starting $12/box

 

Liberty 120

High-speed gel system

See you PCR and genotyping results in 6 min with straight banding technology...