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 Coverslips with poly-L-lysine coating

FAQ: 

  Table of polylysine needed for coating dishes, plates, and coverslips

Cell culture devices

Total coating area per plate

Polylysine needed

6 well culture plate 57cm² 427.5ug polylysine at 50ug/ml
12 well culture plate 45.6cm² 342ug polylysine at 50ug/ml
24 well culture plate 45.6cm² 342ug polylysine at 50ug/ml
48 well culture plate 45.6cm² 342ug polylysine at 50ug/ml
96 well culture plate 30.7cm² 230ug polylysine at 50ug/ml
35mm dish 9cm² 67.5ug polylysine at 50ug/ml
60mm dish 21cm² 157.5ug polylysine at 50ug/ml
100mm dish 55cm² 412.5ug polylysine at 75ug/ml
150mm dish 152cm² 1140ug polylysine at 75ug/ml
245mm dish 500cm² 3750ug polylysine at 100ug/ml
12 mm coverslips round 2.3cm² 17.25ug polylysine at 100ug/ml
15 mm coverslips round 3.5cm² 26.25ug polylysine at 100ug/ml
18 mm coverslips round 5cm² 37.50ug polylysine at 100ug/ml
22 mm coverslips round 7.6cm² 57ug polylysine at 100ug/ml
25 mm coverslips round 9.8cm² 73.50ug polylysine at 100ug/ml

 

Should I dissolve polylysine in dd-water for coating?

  • Poly lysine coating in dd-water is simple but the coating efficiency is low. The pH of dd-water is acidic, usually lower than pH6. After dissolving polylysine, the ph value is further reduced to 3, which is not optimized for poly lysine coating. 

  • Solution: Use high pH polylysine coating, such as pH 8.5, in borate buffer, which enhances poly lysine adsorption to cell culture surface. 

  • Critical: The borate buffer residue must be rinsed away thoroughly from coverslips before cell culture.

How much poly lysine needed to coat my dish / plates / coverslips?

  • High concentration polylysine is recommended. Minimum required amount of poly lysine is 7.5ug/cm² surface area of your culture dish or coverslips. For example, to coat 100 pieces of 18mm diameter coverslips, the total surface area of 100 pieces of coverslips is 0.9x0.9x3.1416 x2x100 = 510cm². The required poly lysine is 510x7.5ug = 3825ug = 3.825mg.

 Products: Poly-L-lysine pre-coated German coverslips for cell & neuron culture

Cat. # Item Description Unit Price
H-12-pll 12mm diameter, # 1 thickness, Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag x 3 bags =45 pieces, ready to use for cell cultures. $54.75
GG-12-pll 12mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $89.50
H-14-pll 14mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $54.75
GG-14-pll 14mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag,  ready to use for cell cultures. $89.50
H-15-pll 15mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $54.75
GG-15-pll 15mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $89.50
H-18-pll 18mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $56.25
GG-18-pll 18mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $92.50
H-18-1.5-pll 18mm diameter, # 1.5 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $61.25
GG-18-1.5-pll 18mm diameter, # 1.5 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $102.50
H-20-pll 20mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $56.25
GG-20-pll 20mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag,  ready to use for cell cultures. $92.50
H-22-pll 22mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $56.25
GG-22-pll 22mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag,  ready to use for cell cultures. $92.50
H-22-1.5-pll 22mm diameter, # 1.5 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $61.25
GG-22-1.5-pll 22mm diameter, # 1.5 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $102.50
H-24-pll 24mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $57.75
GG-24-pll 24mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $95.50
H-25-pll 25mm diameter, # 1 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $59.75
GG-25-pll 25mm diameter, # 1 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $99.50
H-25-1.5-pll 25mm diameter, # 1.5 thickness, 45 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $62.75
GG-25-1.5-pll 25mm diameter, # 1.5 thickness, 90 pieces of Poly L lysine (PLL) coated coverslips, CE certified German coverglass, sterilized by radiation, sealed with15 pieces per bag, ready to use for cell cultures. $105.50

 

Neuvitro Corporation

The poly lysine coating expert  

with G3 poly lysine coating system

 

 

Poly-D-lysine coating

Pre-coated coverslips

Ready to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell culture, starting $55/box

 

Rat tail Collagen

Pre-coated coverslips

Ready to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell culture, starting $65/box

 

Fibronectin coating

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Ready to use German coverslip with fibronectin coating on both sides for cell culture, starting $89/box

 

Laminin coating

Pre-coated coverslips

Ready to use German coverslip with human Laminin coating on both sides for cell culture and neuron culture, starting $94/box

 

Gelatin coating

Pre-coated coverslips

Ready to use German coverslip with porcine gelatin coating on both sides for cell culture, starting $89/box

 

German Coverslips 

Cell culture tested

CE certified, tested for primary cell and primary neuron cultures, starting $12/box

 

Liberty 120

High-speed gel system

See you PCR and genotyping results in 6 min with straight banding technology...