neuVitro
German
coverslip with digital precise polylysine coating
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Professional
polylysine coating procedure for enhanced cell culture
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This
protocol works very well for primary cell culture and primary neuron
culture as shown at right
It
can be adapted to coat culture dishes and plates.
Click
to enlarge neuronal culture
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Wash
coverslips 2 hours on shaker.
Rinse
coverslips 6 times x 30 minutes each on shaker.
Treat
coverslips in 100% Ethanol x 2 hour at room temperature.
Treat
coverslips in 70% concentrated Nitric Acid x 24 hours at room
temperature.
Rinse
coverslips in dd-water 6 times x 1 hour each on
shaker.
Primary
coating, 1mg/ml, 12 hours at room temperature. Rinse
coverslips x 2 times to remove unbound poly lysine
Dry
coverslips 12 hours in laminar hood.
Secondary
coating, 1mg/ml, 12 hours at room temperature.
Rinse
coverslips x 2 times to remove unbound poly lysine
Dry
coverslips 12 hours in laminar hood.
Seal
coated coverslips into bags, 15 units/bag.
Sterilization
by radiation.
Quality
inspection.
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Cell
Culture Coverslips
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CE
certified, tested
for primary cell and primary neuron cultures, starting $12/box
Ready
to use German coverslip with poly-D-lysine (PDL) coating on both sides for cell
culture, starting $55/box
Ready
to use German coverslip with Millipore rat tail type I Collagen coating on both sides for cell
culture, starting $65/box
Ready
to use German coverslip with fibronectin coating on both sides for cell
culture, starting $89/box
Ready
to use German coverslip with human Laminin coating on both sides for cell
culture and neuron culture, starting $94/box
Ready
to use German coverslip with porcine gelatin coating on both sides for cell
culture, starting $89/box
See
you PCR and genotyping results in 6 min with straight banding
technology...
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