google-site-verification: googleeb29447abf615d32.html Coverslips for cell culture | neuronal culture | Neuvitro.com | USA

Coverslips for cell culture

Neuvitro Corporation owns the leading technology to provide professional coating with the broadest coverage of substrates, such as PDL, PLL, PLO, Collagen, Gelatin, Laminin, Fibronectin, PDL+Laminin, PDL + Fibronectin, PLO+Laminin, PLO+Fibronectin, PDL+Laminin+Fibronectin, etc. The majority of coated German coverslips available from Electron Microscopy Science, ThermoFisher Scientific, and VWR is actually manufactured by Neuvitro Corporation. Free samples are available.

Primary neuron cultured on Neuvitro coated coverslips without feedlayer support

List of coverslips for cell culture (1/2 year storage life)

Why use coverslips for cell culture is better than plates and slides?

It is well known that optically clear German coverslips is required to generate crystal clear confocal imaging. Why? The answers are:

  • Cells attached directly on optically clear coverslip which minimizes the distance between cells and the objective lens so that 60x and 100x objective lens can be used for imaging.

  • The distortion of mount medium is avoided because there is no gap between cultured cells and the objective lens. Mount medium liquid is otherwise required to fill the gap between cells and coverslips if the culture were made on slides or multiwell plates.

  • Coverslips provide freedom of experiment setup to fit any pattern as your wish.

 

The importance of coating coverslips for cell culture

Cells do not like glass surface. Most cells attach coverslips loosely and detach from coverslip during after culture processes, such IHC staining. Therefore, a clear layer of cell culture substrate must be used to enhance cell culture attachment on coverslips.

 

The type of coating coverslips for cell culture

Cells are not all the same. Different cells need different culture substrate. Neuvitro is now the only manufacturer in the world capable of providing the broadest range of cell culture substrates, as you can see from the list below:

1.5H 0.170mm +/- 5um High Precision German coverslips for cell culture-imaging

 

Poly-D-Lysine (PDL) coated German coverslips for cell culture-imaging

Poly-L-Lysine (PLL) coated German coverslips for cell culture-imaging

Rat tail Collagen I coated German coverslips for cell culture-imaging

Mouse Laminin coated (on PDL layer) German coverslips for cell culture-imaging

Poly-L-Ornithine Laminin coated German coverslips for stem cell culture-imaging

Fibronectin coated (on PDL layer) German coverslips for cell culture-imaging

Gelatin coated German coverslips for cell culture-imaging

Treated cleaned German glass coverslips with no coating, sterile, ready to use

Round shape German glass coverslips with no coating no treatment for cell culture

Rectangular shape German glass coverslips with no coating no treatment for cell culture

Round shape German glass coverslips for IHC

Rectangular shape German glass coverslips for IHC

Consistancy is critical for image data analysis:

Culture variation is a major problem of invalided data leading to failure of solid conclusion. With Neuvitro advanced coating technology, every single pieces of coverslips were coated uniformly and consistantly to minimize your cell culture variation. Therefore, Neuvitro coverslips can be used as cell culture control in data analysis.

Examples using Neuvitro coverslips for cell culture:

Science
April 30, 2015,
Research Article
Reversible centriole depletion with an inhibitor of Polo-like kinase 4

 

Semin Oncol. 2014 Feb;41(1):93-100. doi: 10.1053/j.seminoncol.2013.12.007. Epub 2013 Dec 12.
Chemotherapy and targeted therapeutics as maintenance of response in advanced non-small cell lung cancer.

Ther Adv Med Oncol. 2014 Jan;6(1):4-15. doi: 10.1177/1758834013510589.
Maintenance treatment after induction therapy in non-small cell lung cancer: latest evidence and clinical implications.

Curr Treat Options Oncol. 2013 Dec;14(4):595-609. doi: 10.1007/s11864-013-0255-3.
Bevacizumab in advanced NSCLC: chemotherapy partners and duration of use

Cancer Res. 2014 Jun 13.
Germline Mutation of Bap1 Accelerates Development of Asbestos-Induced Malignant Mesothelioma.

J Cardiovasc Pharmacol. 2013 Apr;61(4):311-7. doi: 10.1097/FJC.0b013e318280e16e.
Sulfur-containing angiotensin-converting enzyme inhibitor 3-thienylalanine-ornithyl-proline activates endothelial function and expression of genes involved in Renin-Angiotensin system.

Cancer Res. 2014 Mar 1;74(5):1390-403. doi: 10.1158/0008-5472.CAN-13-1275.
LIMD2 is a small LIM-only protein overexpressed in metastatic lesions that regulates cell motility and tumor progression by directly binding to and activating the integrin-linked kinase.

Cell Tissue Res. 2014 May 28.
MicroRNA-dependent genetic networks during neural development.


Oxford Journals Volume 23 Issue I, P145-156

Research Article
Hexokinase activity is required for recruitment of parkin to depolarized mitochondria


Cancer Research, June 1, 2014
Fatty acid-binding protein E-FABP restrcts tumor growth by promoting IFN-b responses in tumor-associated macrophages

1.5H high performance coverslips

 

Molecular Reproduction and Development

Volume 81, Issue 12

Abstract
Kpna7 interacts with egg-specific nuclear factors in the rainbow trout (oncorhynchus mykiss)

More leteratures

 

BMC Immunology, 

May 12, 2015
Research Article
Targeting epidermal fatty acid binding protein for treatment of experimental autoimmune encephalomyelitis

 

Journal of Cell Biology
2014, 206: 923-936
Research Article
A matrix metallopreteinase mediates long-distance attenuation of stem cell proliferation

 

Nature
Article #6211, doi: 10.1038, Sept 5 2014

Optical assay of erythrocyte function in banked blood

 

Nature
May 15, 2015,
Scientific report
Number of nanoparticles per cell through a spectrophotometric method- A key parameter to assess nanoparticle-based cellular assays

 

The Journal of Biological Chemistry
Feb. 6, 2015
Research Article

4E-BPs control fat storage by regulating the expresion of Egr1 and ATGL

 

EMBO-Molecular Systems Biology
2014, 206: 923-936
Research Article
Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation

 

The Journal of Clinical Investigation
May 26, 2015
Research Article
Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase 1

 

Molecular Biology of The Cell
June 30, 2014
Research Article

FUS is sequestered in nuclear aggregates in ALS patient fibroblasts

Journal of Biomedical Materials Research

 

The Journal of Clinical Investigation
April 27 , 2015,
Research Article
Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase-1

 

Symbiosis
May 20, 2015,
Research Article

Topiramete protects pericytes from glucotoxicity: Rle for mitochondrial CA VA in cerebromicrovascular disease in diabetes

 

Jan 28, 2015
Special report
Adhesion and activation of Platelets from subjects with coronary artery disease and apparently healthy individuals on biomaterials

 

Molecular Cellular Biology
doi: 10.1128 / MCB.01630-13
Research Article
Activation of stress response pathways promote formation of antiviral granules and restrct virus replication

 

Journal of Cell Biology
2014, 204: 559-573
Research Article
Aggregation state determines the localization and function of M1-and M23-aquaporin-4 in astrocytes


Biotechniques

November, 2014

Report
Marking cells with infrared fluorescent proteins to preserve photoresponsiveness in the retina

 

Methods
Volume 66, Issue 3, 1 April 2014, Pages 370–379
Developmental Biology
Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging


Journal of Marine Biology
Volume 2014 (2014), Article ID 769356, 11 pages
http://dx.doi.org/10.1155/2014/769356
Research Article
The Immune Response of Acanthaster planci to Oxbile Injections and Antibiotic Treatment


Am J Cancer Res 2013;3(3):278-289
www.ajcr.us /ISSN:2156-6976/ajcr0000201
The C-terminal common to group 3 POTES (CtG3P): a 
newly discovered nucleolar marker associated with 
malignant progression and metastasis

Nature America Inc. • http://biotech.nature.co
Molecular breeding of carotenoidbiosynthetic pathways

J Biol Chem. Apr 11, 2014; 289(15): 10930–10938.
Published online Feb 25, 2014. doi: 10.1074/jbc.M113.533216
PMCID: PMC4036204
The DDN Catalytic Motif Is Required for Metnase Functions in Non-homologous End Joining (NHEJ) Repair and Replication Restart

Transl Psychiatry. Nov 2013; 3(11): e323.
Published online Nov 5, 2013. doi: 10.1038/tp.2013.96
PMCID: PMC3849963
Derivation of neural stem cells from an animal model of psychiatric disease

Experimental and Molecular Pathology
Available online 30 July 2014
In Press
Systemic distribution, subcellular localization and differential expression of sphingosine-1-phosphate receptors in benign and malignant human tissues

Journal of Advanced Research
Available online 22 May 2014
In Press
ORIGINAL ARTICLE
Expression, genetic localization and phylogenic analysis of NAPlr in piscine Streptococcus dysgalactiae subspecies dysgalactiae isolates and their patterns of adherence

ORIGINAL RESEARCH ARTICLE Front. Immunol., 17 September 2013 | doi: 10.3389/fimmu.2013.00287
Th1/M1 conversion to Th2/M2 responses in models of inflammation lacking cell death stimulates maturation of monocyte precursors to fibroblasts

Hindawi Publishing Corporation
Journal of Marine Biology
Volume 2014, Article ID 769356, 11 pages
http://dx.doi.org/10.1155/2014/769356
Research Article
The Immune Response of Acanthaster planci to
Oxbile Injections and Antibiotic Treatment

McMaster University © by Dipannita Basu, July 2013
CHARACTERIZATION OF THE MECHANISM OF ACTION FOR NOVEL 
ALLOSTERIC MODULATORS OF THE DOPAMINE D2 RECEPTOR

J CARDIOVASC PHARMACOL THER 2013 18: 280 originally published online 14 December 2012
Probenecid as a Noninjurious Positive Inotrope in an Ischemic Heart Disease Murine Model

Stem Cell Reviews and Reports
August 2014
Date: 05 Aug 2014
Motor Neuron Differentiation from Pluripotent Stem Cells and Other Intermediate Proliferative Precursors that can be Discriminated by Lineage Specific Reporters poly-d-lysine coating coverslips


The Royal Society of Chemistry
2015,
Research Article
Cationic Lipsomes as efficient nanocarriers for the drug delivery of an anticancer cholesterol-based ruthenium complex


Carroll College Natural Sciences Department
Helena, Montana
Exploring the Role of Olfm1 in the Trafficking of GluR2- containing AMPA Receptors

J Neurosci. May 22, 2013; 33(21): 9169–9175.
doi: 10.1523/JNEUROSCI.0301-13.2013
The SNARE proteins SNAP25 and synaptobrevin are involved in endocytosis at hippocampal synapses

Nature Communications 5, Article number: 3356 doi:10.1038/ncomms4356
Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles

Nat Commun. 2014 Jun 17;5:4112. doi: 10.1038/ncomms5112.
Senescence impairs direct conversion of human somatic cells to neurons.
More leteratures

Nat Neurosci. 2014 Feb;17(2):232-9. doi: 10.1038/nn.3615. Epub 2014 Jan 5.
Presynaptic glycine receptors as a potential therapeutic target for hyperekplexia disease.

J Natl Compr Canc Netw 2014;12:889-897
Optimal First-Line and Maintenance Treatments for Advanced-Stage Nonsquamous Non-Small Cell Lung Cancer
 

 

back