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Enhance Neuron growth and transfection in primary neurons and neuronal cell lines

Do you satisfy the efficiency of your neuron cell transfection protocol?

If not, have you thought about the main cause of your poor transfection efficiency?

 

The problem of current cell transfection protocols

Numerous leading companies have claimed their transfection reagents and kits are the best. Then why the best neuron transfection reagents from the best companies used by the best laboratories have failed to solve the difficulties of primary neuron transfections? 

 

The main cause of neuron cell transfection  failure:

Attentions have been wrongly focused!

Most of your transfected primary neurons have died after your transfection protocol! 

Neuron transfection reagent plays ONLY a minor role in the success of high efficiency neuron transfection!

Scientists at neuvitro have conducted neuron culture of primary neurons and neuronal cell lines for over 10 years and developed protocols to generate robust neuron growth. While in testing a variety of different cell transfection kits and reagents with our primary neuronal culture, it was found that the transfection efficiency in Neuvitro protocol is always significantly higher than in manfuacturer's protocol.

 

The Neuvitro finding:

The key to success is creating energized primary neuron culture system before and after cell transfection so that damaged neurons by transfection reagents can regain their health rapidly afterward so that the majority of your primary neurons can survive your torture of external insertions. It means you will see a robust increase in your transfection efficiency easily, even using simple calcium precipitation method.

Below is the List of coated coverslips for promoting neuron growth and viral transfection in culture of primary neurons and neuronal cell lines:

GG-25-pll, neuronal culture tested, 25mm diameter round, #1 thickness, 90 pieces of German coverslip with Poly-L-lysine coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$99.50

GG-25-1.5-pll, neuronal culture tested, 25mm diameter round, #1.5 thickness, 90 pieces of German coverslip with Poly-L-lysine coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$125.50

GG-25-pdl, neuronal culture tested, 25mm diameter round, #1 thickness, 90 pieces of German coverslip with Poly-D-lysine coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$105.50

GG-25-1.5-pdl, neuronal culture tested, 25mm diameter round, #1.5 thickness, 90 pieces of German coverslip with Poly-D-lysine coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$135.50

GG-25-laminin, tested and coated for neuron growth and viral transfection in culture of primary neurons and neuronal cell lines: 25mm diameter round, #1 thickness, 60 pieces of German coverslip with PDL and Laminin coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$149.65

GG-25-1.5-laminin, tested and coated for neuron growth and viral transfection in culture of primary neurons and neuronal cell lines:   25mm diameter round, #1.5 thickness, 60 pieces of German coverslip with PDL and Laminin coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$179.65

GG-25-fibronectin, neuronal culture tested, 25mm diameter round, #1 thickness, 60 pieces of German coverslip with PDL and Fibronectin coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$144.65

GG-25-1.5-fibronectin,  neuronal culture tested, 25mm diameter round, #1 thickness, 60 pieces of German coverslip with PDL and Fibronectin coating on both sides for cell culture and neuron culture, sterilized by radiation, sealed in 15 pieces/bag, ready to use.$174.65

GG-22-pll,  neuronal culture tested, 22mm diameter round, #1 thickness, 90 pieces of PLL-coated coverslips, sterilized by radiation, sealed in 15 pieces/bag,  ready to use German coverslip glass for cell and neuron cultures$92.50

GG-22-1.5-pll,  neuronal culture tested, 22mm diameter round, #1.5 thickness, 90 pieces of PLL-coated coverslips, sterilized by radiation, sealed in 15 pieces/bag,  ready to use German coverslip glass for cell and neuron cultures$102.50

GG-22-pdl,  neuronal culture tested, 22mm diameter round, #1 thickness, 90 pieces of PDL-coated coverslips, sterilized by radiation, sealed in 15 pieces/bag, ready to use German coverslip glass for cell and neuron cultures$102.50

GG-22-1.5-pdl, neuronal culture tested, 22mm diameter round, #1.5 thickness, 90 pieces of PDL-coated coverslips, sterilized by radiation, sealed in 15 pieces/bag, ready to use German coverslip glass for cell and neuron cultures$112.50

GG-22-laminin,  neuronal culture tested, 22mm diameter round, #1 thickness, 60 pieces of German coverslips coated with natural human laminin on top of poly-d-lysine layer, sterilized by radiation, sealed 15 pieces/bag, ready to use German coverslips for cell and neuron cultures$129.65

GG-22-1.5-laminin,  neuronal culture tested, 22mm diameter round, #1.5 thickness, 60 pieces of German coverslips coated with natural human laminin on top of poly-d-lysine layer, sterilized by radiation, sealed 15 pieces/bag, ready to use German coverslips for cell and neuron cultures$139.65

GG-22-fibronectin,  neuronal culture tested, 22mm diameter round, #1 thickness, 60 pieces of German coverslips coated with fibronectin on top of poly-d-lysine layer, sterilized by radiation, sealed 15 pieces/bag, ready to use German coverslip glass for cell and neuron cultures$124.65

GG-22-1.5-fibronectin,  neuronal culture tested, 22mm diameter round, #1.5 thickness, 60 pieces of German coverslips coated with fibronectin on top of poly-d-lysine layer, sterilized by radiation, sealed 15 pieces/bag, ready to use German coverslip glass for cell and neuron cultures$134.65 back